[I]. Laboratory:

DNA extraction from parasites and different biological materials, PCR, Gel and PCR purification, Southern blotting, Restriction analysis, DNA cloning, generation of construct. Screening of cDNA library using radioactive and non-radioactive methods, Sequencing and Site-directed mutagenesis. Sequence analysis using Vector NTI software. Facs analysis of DNA content during different phases of the cell cycle. Histone HI kinase assay. Recombinant protein production & purification on Ni-NTA resin and Bio-rad FLPC machine, Western blot analysis, Protein analysis and visualization on SDS-PAGE. Culture of: Cell line, Leishmania, T. evansi and Plasmodium parasites. Identification of mosquitos species.Mass Spectrometry (MS- MALDI-TOF): Preparation of sample, enzymatic digestion, In-gel digestion, peptide purification, Protein Identification of Mass spectrometry data using MASCOT software. RT-PCR, nested – PCR, Real Time – PCR, Virus culturing, SeqMan sequence analysis software.

[II]. Computer:

• Fundamentals of computers and networks, MS-DOS, Windows 9x, 2000, XP. Microsoft Office, Word, LOTUS123, D-BASEIII⁺, Excel, PowerPoint.
• Netscape Communicator, Microsoft Internet Explorer.
• SPSS statistical program.
• Internet regular user mainly for information search and email.

[III]. Bioinformatics:

• Primers designing, Restriction site analysis.
• Sequence Analysis and comparison (BLAST – CLUSTALW/X).
• Phylogenetic Analysis.
• Vector NTI software package.
• Molecular Biology Computing; GCG Usage.
• Protein structure prediction.
• 3D modeling of protein structures.
• Genomics: Gene Prediction, Assembly, Annotation, Comparative Genomics.
• EST clustering.
• Similarity Searches.
• Familiar with Plasmodium Data Base, PlasmoDB, MR4.
• Familiar with VectorBase Data Base.
• Familiar with TriTrypDB, Kinetoplastid Genomic Resources.
• MASCOT software for identification of protein sequence.
• SeqMan analysis for sequences