Molecular Biology Related Skills

  • Western Blotting
  • Strong Background in Transient Transfection of Mammalian Cells 3- The Polymerase Chain Reaction, RT-PCR
  • Restriction Enzyme Digests
  • Various Cell Culture Techniques: HEK293T (Human Embryonic Kidney T- Cells), HUVEC (Human Umbilical Vein Endothelial Cells), HUVEC/TERT2 Cells, A549 Lung cancer cell lines, HeLa (cervical cancer cells) & CRISPR Cas9 Knockout HeLa Cell Lines
  • RNA Extraction
  • Immuno-precipitation Techniques
  • Plasmid Preparation (Mini, Midi, Maxi Prep.) 9- Bacterial Transformation
  • Gene Knock-Down Techniques
  • Background in flow-cytometric analysis of mammalian cells (FACS of HEK293T Cells: Human Embryonic Kidney T-Cells)
  • Immunofluorescence Techniques
  • Fluorescent in Situ Hybridization targeting Ribonucleic Acid Molecules (RNA FISH)
  • Preparing different buffers in the laboratory: TBS, TBST, SDS, Western Blot & protein lysis related buffers
  • Preparing bacterial media
  • Maintaining bacterial stocks /plasmids.