• I- Laboratory
  • II – Genetic Analysis:
  • III – Bioinformatics
  • IV – Computer

I- Laboratory

  • DNA extraction from different biological material.
  • RNA extraction.
  • PCR, ARMS –PCR.
  • RFLP.
  • Southern blotting
  • Gel and PCR purification.
  • Microsatelite, analysis-using Gene scan -Genotyper softwares.
  • Cloning.
  • Generation of construct.
  • Muagensis.
  • Transfection.
  • Luciferase reporter gene assay.
  • Real Time PCR.
  • Sequencing.
  • Stimulation assay.
  • Culture of: Cell line. Peripheral blood Mononuclear Cell (PBMC). Malaria and leishmania parasite.

II – Genetic Analysis:

  • Pedigree inconsistency using Pedcheck.
  • Association analysis using; TDT, TDTphase.
  • Linkage Disequilibrium Hardy-Weinberg deviation analysis using Haploview.
  • Linkage analysis using SPLINK, ALLEGRO

III – Bioinformatics

  • Primers designing, Restriction site analysis, sequence analysis using Sequence Navigator and Standen package (pregap, gap4).
  • Sequences alignment (Blast). Alibaba and MatInspector, for identification of putative Transcription site.
  • Multiple sequence alignment using Bioedit and Clustal W
  • Ensemble, NCBI and Hapmap Genome Browser, for gene annotation

IV – Computer

  • Windows, UNIX and Linux, Microsoft Office, Word, Excel, power point, Epinfo, picture editing programs, SPSS programs.
  • Internet and email regular user mainly for scientific information.