- I- Laboratory
- II – Genetic Analysis:
- III – Bioinformatics
- IV – Computer
I- Laboratory
- DNA extraction from different biological material.
- RNA extraction.
- PCR, ARMS –PCR.
- RFLP.
- Southern blotting
- Gel and PCR purification.
- Microsatelite, analysis-using Gene scan -Genotyper softwares.
- Cloning.
- Generation of construct.
- Muagensis.
- Transfection.
- Luciferase reporter gene assay.
- Real Time PCR.
- Sequencing.
- Stimulation assay.
- Culture of: Cell line. Peripheral blood Mononuclear Cell (PBMC). Malaria and leishmania parasite.
II – Genetic Analysis:
- Pedigree inconsistency using Pedcheck.
- Association analysis using; TDT, TDTphase.
- Linkage Disequilibrium Hardy-Weinberg deviation analysis using Haploview.
- Linkage analysis using SPLINK, ALLEGRO
III – Bioinformatics
- Primers designing, Restriction site analysis, sequence analysis using Sequence Navigator and Standen package (pregap, gap4).
- Sequences alignment (Blast). Alibaba and MatInspector, for identification of putative Transcription site.
- Multiple sequence alignment using Bioedit and Clustal W
- Ensemble, NCBI and Hapmap Genome Browser, for gene annotation
IV – Computer
- Windows, UNIX and Linux, Microsoft Office, Word, Excel, power point, Epinfo, picture editing programs, SPSS programs.
- Internet and email regular user mainly for scientific information.