HPLC-UV was used throughout the course of the study as stability-indicating method.
The central composite design has been selected to predict the effect of factors on the characteristics of the produced liposomes and the encapsulation efficiency of the drug within the vesicles bilayers. The software statistical package Minitab® was employed to build up the design, analyse data and draw the response surfaces. HPLC-UV was used to determine the amount of the entrapped drug and the physical properties of the manufactured liposomes in terms of size and size distribution were measured using dynamic light scattering (DLS) method.
Gained Skills: