{"id":152,"date":"2017-12-28T12:58:06","date_gmt":"2017-12-28T12:58:06","guid":{"rendered":"http:\/\/staffpages.uofk.edu\/nahla-osman\/?page_id=152"},"modified":"2017-12-28T12:58:06","modified_gmt":"2017-12-28T12:58:06","slug":"experiences-skills","status":"publish","type":"page","link":"https:\/\/staffpages.uofk.edu\/nahla-osman\/?page_id=152","title":{"rendered":"Experiences &#038; skills"},"content":{"rendered":"<p><span style=\"font-size: 12pt; font-family: times new roman, times;\"><strong>[I]. Laboratory:<\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt; font-family: times new roman, times;\">DNA extraction from parasites and different biological materials, PCR, Gel and PCR purification, Southern blotting, Restriction analysis, DNA cloning, generation of construct. Screening of cDNA library using radioactive and non-radioactive methods, Sequencing and Site-directed mutagenesis. Sequence analysis using Vector NTI software. Facs analysis of DNA content during different phases of the cell cycle. Histone HI kinase assay. Recombinant protein production &amp; purification on Ni-NTA resin and Bio-rad FLPC machine, Western blot analysis, Protein analysis and visualization on SDS-PAGE. Culture of: Cell line, <em>Leishmania<\/em>, <em>T. evansi <\/em>and <em>Plasmodium<\/em> parasites. Identification of mosquitos species.Mass Spectrometry (MS- MALDI-TOF): Preparation of sample, enzymatic digestion, In-gel digestion, peptide purification, Protein Identification of Mass spectrometry data using MASCOT software. RT-PCR, nested \u2013 PCR, Real Time \u2013 PCR, Virus culturing, SeqMan sequence analysis software.<\/span><\/p>\n<p><span style=\"font-size: 12pt; font-family: times new roman, times;\"><strong>[II]. Computer:<\/strong><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Fundamentals of computers and networks, MS-DOS, Windows 9x, 2000, XP. Microsoft Office, Word, LOTUS123, D-BASEIII<sup>+<\/sup>, Excel, PowerPoint.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Netscape Communicator, Microsoft Internet Explorer.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">SPSS statistical program.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Internet regular user mainly for information search and email.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 12pt; font-family: times new roman, times;\"><strong>[III]. Bioinformatics:<\/strong><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Primers designing, Restriction site analysis.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Sequence Analysis and comparison (BLAST \u2013 CLUSTALW\/X).<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Phylogenetic Analysis.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Vector NTI software package.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Molecular Biology Computing; GCG Usage.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Protein structure prediction.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">3D modeling of protein structures.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Genomics: Gene Prediction, Assembly, Annotation, Comparative Genomics.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">EST clustering.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Similarity Searches.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Familiar with Plasmodium Data Base, PlasmoDB, MR4.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Familiar with VectorBase Data Base.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">Familiar with TriTrypDB, Kinetoplastid Genomic Resources.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">MASCOT software for identification of protein sequence.<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: times new roman, times;\">SeqMan analysis for sequences<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>[I]. Laboratory: DNA extraction from parasites and different biological materials, PCR, Gel and PCR purification, Southern blotting, Restriction analysis, DNA [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"page-publications.php","meta":{"footnotes":""},"class_list":["post-152","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/staffpages.uofk.edu\/nahla-osman\/index.php?rest_route=\/wp\/v2\/pages\/152","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/staffpages.uofk.edu\/nahla-osman\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/staffpages.uofk.edu\/nahla-osman\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/staffpages.uofk.edu\/nahla-osman\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/staffpages.uofk.edu\/nahla-osman\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=152"}],"version-history":[{"count":1,"href":"https:\/\/staffpages.uofk.edu\/nahla-osman\/index.php?rest_route=\/wp\/v2\/pages\/152\/revisions"}],"predecessor-version":[{"id":154,"href":"https:\/\/staffpages.uofk.edu\/nahla-osman\/index.php?rest_route=\/wp\/v2\/pages\/152\/revisions\/154"}],"wp:attachment":[{"href":"https:\/\/staffpages.uofk.edu\/nahla-osman\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=152"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}