1-Molecular Biology and Recombinant DNA techniques
DNA and RNA extraction from different biological materials, PCR, RT-PCR., RFLP, Hybridization techniques Real Time PCR, and DNASequencing. DNA cloning, generation of transfection constructs, mutagenesis techniques, knock out of essential genes, gene replacement and transfection of Plasmodium parasites.Cell and parasite culture, Hybridoma cell culture, malaria and leishmania parasite in vitro culture. Experience in cell line culture, culturing peripheral blood mononuclear cells (PBMC).
2- Biochemistry (Proteomics)
Expression of genes in E. coli and Pichia pastoris. Fermentation and Purfication of Proteins.
Purification of proteins using Nickel IMAC affinity chromatography and size exclusion. Chromatography. SDS-Gel Analysis. Western blotting technique. Immunofluorescence assay.
3- Immunology
Design and production of proteins in the methylotrophic yeast P. pastoris. Antigen and vaccine preparation. Small animals immunization. Purification of immunoglobulins and affinity chromatography. ELISAs (indirect ELISA/competition ELISA/avidity ELISA). Cytokine ELISA. Agglutantion and precipitation tests. Parasite growth inhibition assay. Immuno blotting Flowcytometry (FACs).
4- Laboratory animals
Experience in working with mice, rabbits and rhesus monkeys.
5- Bioinformatics
Primers design, DNA sequence restriction site analysis, DNA sequence analysis. Sequences alignment (BLAST). Alibaba software, for identification of putative transcription site. Ensembl Genome browser, NCBI and Genome Browser for gene annotation. Experiece with Haploview/Bioedit/Arlequine3/phylip and R softwares.
6- Computer skills
Have good command of the Windows, UNIX and Linux, Microsoft Office tools (Word, Excel, and PowerPoint), Epinfo, SPSS V 20.0, GraphPad Prism. Experienced in R statistical language, computing and analysis. Experienced in Graphic design applications (adobe photoshop and Graph-pad prism). Internet regular user mainly for information search and email.
