{"id":60,"date":"2023-02-02T12:54:04","date_gmt":"2023-02-02T12:54:04","guid":{"rendered":"http:\/\/staffpages.uofk.edu\/intisar-fouad\/?page_id=60"},"modified":"2023-02-02T12:54:04","modified_gmt":"2023-02-02T12:54:04","slug":"molecular-biology-related-skills","status":"publish","type":"page","link":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/?page_id=60","title":{"rendered":"Molecular Biology Related Skills"},"content":{"rendered":"<ul>\n<li><span style=\"font-family: times new roman, times, serif;\">Western Blotting<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Strong Background in Transient Transfection of Mammalian Cells 3- The Polymerase Chain Reaction, RT-PCR<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Restriction Enzyme Digests<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Various Cell Culture Techniques: HEK293T (Human Embryonic Kidney T- Cells), HUVEC (Human Umbilical Vein Endothelial Cells), HUVEC\/TERT2 Cells, A549 Lung cancer cell lines, HeLa (cervical cancer cells) &amp; CRISPR Cas9 Knockout HeLa Cell Lines<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">RNA Extraction<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Immuno-precipitation Techniques<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Plasmid Preparation (Mini, Midi, Maxi Prep.) 9- Bacterial Transformation<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Gene Knock-Down Techniques<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Background in flow-cytometric analysis of mammalian cells (FACS of HEK293T Cells: Human Embryonic Kidney T-Cells)<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Immunofluorescence Techniques<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Fluorescent in Situ Hybridization targeting Ribonucleic Acid Molecules (RNA FISH)<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Preparing different buffers in the laboratory: TBS, TBST, SDS, Western Blot &amp; protein lysis related buffers<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Preparing bacterial media<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Maintaining bacterial stocks \/plasmids.<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Western Blotting Strong Background in Transient Transfection of Mammalian Cells 3- The Polymerase Chain Reaction, RT-PCR Restriction Enzyme Digests Various [&hellip;]<\/p>\n","protected":false},"author":2038,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-publications.php","meta":{"footnotes":""},"class_list":["post-60","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/index.php?rest_route=\/wp\/v2\/pages\/60","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/index.php?rest_route=\/wp\/v2\/users\/2038"}],"replies":[{"embeddable":true,"href":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=60"}],"version-history":[{"count":1,"href":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/index.php?rest_route=\/wp\/v2\/pages\/60\/revisions"}],"predecessor-version":[{"id":62,"href":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/index.php?rest_route=\/wp\/v2\/pages\/60\/revisions\/62"}],"wp:attachment":[{"href":"https:\/\/staffpages.uofk.edu\/intisar-fouad\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=60"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}