{"id":60,"date":"2014-10-15T09:44:03","date_gmt":"2014-10-15T09:44:03","guid":{"rendered":"http:\/\/staffpages.uofk.edu\/hiba-salaheldin\/?page_id=60"},"modified":"2014-10-15T09:44:13","modified_gmt":"2014-10-15T09:44:13","slug":"experiences-and-skills","status":"publish","type":"page","link":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/?page_id=60","title":{"rendered":"Experiences and Skills"},"content":{"rendered":"<ul>\n<li>I- Laboratory<\/li>\n<li>II &#8211; Genetic Analysis:<\/li>\n<li>III &#8211; Bioinformatics<\/li>\n<li>IV &#8211; Computer<\/li>\n<\/ul>\n<h2><strong>I- Laboratory<\/strong><\/h2>\n<ul>\n<li>DNA extraction from different biological material.<\/li>\n<li>RNA extraction.<\/li>\n<li>PCR, ARMS \u2013PCR.<\/li>\n<li>RFLP.<\/li>\n<li>Southern blotting<\/li>\n<li>Gel and PCR purification.<\/li>\n<li>Microsatelite, analysis-using Gene scan -Genotyper softwares.<\/li>\n<li>Cloning.<\/li>\n<li>Generation of construct.<\/li>\n<li>Muagensis.<\/li>\n<li>Transfection.<\/li>\n<li>Luciferase reporter gene assay.<\/li>\n<li>Real Time PCR.<\/li>\n<li>Sequencing.<\/li>\n<li>Stimulation assay.<\/li>\n<li>Culture of: Cell line. Peripheral blood Mononuclear Cell (PBMC). Malaria and <em>leishmania<\/em> parasite.<\/li>\n<\/ul>\n<h2><strong>II &#8211; Genetic Analysis:<\/strong><\/h2>\n<ul>\n<li>Pedigree inconsistency using Pedcheck.<\/li>\n<li>Association analysis using; TDT, TDTphase.<\/li>\n<li>Linkage Disequilibrium Hardy-Weinberg deviation analysis using Haploview.<\/li>\n<li>Linkage analysis using SPLINK, ALLEGRO<\/li>\n<\/ul>\n<h2>III &#8211; Bioinformatics<\/h2>\n<ul>\n<li>Primers designing, Restriction site analysis, sequence analysis using Sequence Navigator and Standen package (pregap, gap4).<\/li>\n<li>Sequences alignment (Blast). Alibaba and MatInspector, for identification of putative Transcription site.<\/li>\n<li>Multiple sequence alignment using Bioedit and Clustal W<\/li>\n<li>Ensemble, NCBI and Hapmap Genome Browser, for gene annotation<\/li>\n<\/ul>\n<h2><strong>IV &#8211; Computer<\/strong><\/h2>\n<ul>\n<li>Windows, UNIX and Linux, Microsoft Office, Word, Excel, power point, Epinfo, picture editing programs, SPSS programs.<\/li>\n<li>Internet and email regular user mainly for scientific information.<\/li>\n<\/ul>\n<div id=\"__if72ru4sdfsdfruh7fewui_once\"><\/div>\n<div id=\"__hggasdgjhsagd_once\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>I- Laboratory II &#8211; Genetic Analysis: III &#8211; Bioinformatics IV &#8211; Computer I- Laboratory DNA extraction from different biological material. [&hellip;]<\/p>\n","protected":false},"author":819,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-60","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/index.php?rest_route=\/wp\/v2\/pages\/60","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/index.php?rest_route=\/wp\/v2\/users\/819"}],"replies":[{"embeddable":true,"href":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=60"}],"version-history":[{"count":1,"href":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/index.php?rest_route=\/wp\/v2\/pages\/60\/revisions"}],"predecessor-version":[{"id":62,"href":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/index.php?rest_route=\/wp\/v2\/pages\/60\/revisions\/62"}],"wp:attachment":[{"href":"https:\/\/staffpages.uofk.edu\/hiba-salaheldin\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=60"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}