{"id":56,"date":"2023-01-18T14:05:51","date_gmt":"2023-01-18T14:05:51","guid":{"rendered":"http:\/\/staffpages.uofk.edu\/enaam-hussein\/?page_id=56"},"modified":"2023-01-18T14:05:51","modified_gmt":"2023-01-18T14:05:51","slug":"previous-research-experience","status":"publish","type":"page","link":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/?page_id=56","title":{"rendered":"Previous research experience"},"content":{"rendered":"<ul>\n<li><span style=\"font-family: times new roman, times, serif;\">Dealing with different sampling techniques; blood, cell lines and tissue samples either fresh or Formalin fixed Paraffin embedded.<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Transfection of different cell lines using lipofectamins and Neon electroporation transfection system.<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Analysis and results interpretation of different functional assays including, cell viability and cell count, Annexin v and dead cell assay, cell cycle assay, using Muse instrument from Millipore company.<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Dealing with primary murine Chronic Lymphocytic Leukemia (CLL) cells and cell lines of both suspended and adherent cells (B cell lines and Hepatocellular carcinoma cell lines).<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Recombinant DNA technology, capability of producing stable clones.<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Preparation of co-culture condition that may mimic the Microenvironment of chronic lymphocytic leukaemia, used for drug screening.<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Nucleic acids extraction: DNA &amp; RNA Extraction, protein purification and immune-blotting techniques (blood, cell lines, and tissues).<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Basic and advanced molecular biology techniques (conventional PCR, RT-PCR, Real time-qPCR, digital PCR (ddPCR)).<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Data analysis using Microsoft Excel and GraphPad prism 6.<\/span><\/li>\n<li><span style=\"font-family: times new roman, times, serif;\">Endnote x7 and Mendeley softwares for writing and reference citationand Docear software of mind mapping and reference citation.<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Dealing with different sampling techniques; blood, cell lines and tissue samples either fresh or Formalin fixed Paraffin embedded. Transfection of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-publications.php","meta":{"footnotes":""},"class_list":["post-56","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/index.php?rest_route=\/wp\/v2\/pages\/56","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=56"}],"version-history":[{"count":1,"href":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/index.php?rest_route=\/wp\/v2\/pages\/56\/revisions"}],"predecessor-version":[{"id":58,"href":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/index.php?rest_route=\/wp\/v2\/pages\/56\/revisions\/58"}],"wp:attachment":[{"href":"https:\/\/staffpages.uofk.edu\/enaam-hussein\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=56"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}