Dealing with different sampling techniques; blood, cell lines and tissue samples either fresh or Formalin fixed Paraffin embedded.
Transfection of different cell lines using lipofectamins and Neon electroporation transfection system.
Analysis and results interpretation of different functional assays including, cell viability and cell count, Annexin v and dead cell assay, cell cycle assay, using Muse instrument from Millipore company.
Dealing with primary murine Chronic Lymphocytic Leukemia (CLL) cells and cell lines of both suspended and adherent cells (B cell lines and Hepatocellular carcinoma cell lines).
Recombinant DNA technology, capability of producing stable clones.
Preparation of co-culture condition that may mimic the Microenvironment of chronic lymphocytic leukaemia, used for drug screening.
Nucleic acids extraction: DNA & RNA Extraction, protein purification and immune-blotting techniques (blood, cell lines, and tissues).
Basic and advanced molecular biology techniques (conventional PCR, RT-PCR, Real time-qPCR, digital PCR (ddPCR)).
Data analysis using Microsoft Excel and GraphPad prism 6.
Endnote x7 and Mendeley softwares for writing and reference citationand Docear software of mind mapping and reference citation.
